Validation of otolith microincrements in lab-cultured Paralichthys adspersus (Steindachner, 1867)
Keywords:
Paralichthys adspersus, sagittal, validation, microincrementsAbstract
In Paralichthys adspersus cultivated in the laboratory, the periodicity of formation of the microincrements in their otoliths was validated. Eggs were obtained through hormonal induction and incubated in conical tanks of 150 L. The larvae were collected in 9 x 19 cm polypropylene bags and preserved in 96% ethyl alcohol. The initial sample consisted of 110 specimens and otoliths (sagittal) were extracted from only 59 specimens by using tweezers and a stereoscopic microscope; then, they were washed with distilled water, dried, and stored in black-bottomed cardboard boxes. The otoliths were mounted in slides on a drop of bonded glass, polished with a 1200 grade sandpaper until the nucleus and microincrements were visible, then a 0.3-micron grit abrasive was applied for the finishes. The microincrement counts (three readings) were made from the first ring formed after the nucleus, using a 400X microscope. The degree of accuracy of the counts was obtained. A linear equation was used to determine the periodicity of the microincrement formation where the variables: “known age versus the number of microincrements” and a statistical test for the slope was related. It is concluded that the period of microincrement formation is daily.
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