Evaluation of the pathogenicity of Vibrio sp. strains containing pirA and pirB genes isolated from cultured Penaeus vannamei
Keywords:
Penaeus vannamei, AHPND, experimental infection, histopathologyAbstract
Vibrio species are considered the major bacterial pathogens affecting shrimps. It has been reported that acute hepatopancreatic necrosis disease (AHPND) is caused by different strains of Vibrio parahaemolyticuswith pirA and pirB genes. In this paper, we isolated five V. parahaemolyticus strains that were positive by PCR for the genes pirA and pirB, from shrimps cultured in the Tumbes region. We detected that two strains produce mortality and histological damage in the hepatopancreas, characteristic of AHPND, through experimental infection assays with four of these strains in 1.8 ±0.3 g shrimps. Nevertheless, we also observed that although the two remaining strains are positive by PCR for the genes pirA and pirB, they are not pathogenic in shrimps. These results indicate that V. parahaemolyticus strains present in the shrimp culture from the Tumbes region have different patterns of pathogenicity despite being positive by PCR for the genes pirA and pirB. This suggests that there are bacteria with additional factors to the genes pirA and pirB to cause AHPN.
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