Variaciones genotípicas del Virus del Síndrome de las Manchas Blancas (VSMB) aislados en langostinos de cultivo de Perú
DOI:
https://doi.org/10.53554/boletin.v39i1.407Palabras clave:
Virus del síndrome de las manchas blancas, ORFs, Genotipos, Litopenaeus vannameiResumen
El virus del síndrome de las manchas blancas (VSMB), es uno de los principales patógenos de langostinos peneidos reportado a nivel mundial. Desde su primera aparición hasta la actualidad, el VSMB ha mostrado una notable capacidad para alterar su estructura genética, lo que ha dado lugar a diversidad de genotipos. Con el objetivo de detectar las variantes genotípicas del VSMB presentes en el cultivo de langostinos de la costa norte del Perú (Región Tumbes), en esta investigación, se evaluaron 89 muestras de branquias y postlarvas de langostinos infectados con el VSMB que fueron colectados entre los años 2014 y 2021. El análisis fue realizado mediante la PCR (reacción en cadena de la polimerasa) y secuenciamiento de regiones variables del ADN, ubicados en los marcos de lectura abierta (ORFs= open reading frame) 75, 94, 125, 14/15, 23/24 y un gen transposasa presuntiva. En comparación con los genomas de referencia del VSMB, todas las muestras analizadas presentaron deleciones de 5138 pb en el ORF 14/15, de 11110 pb en el ORF 23/24 y de 1338 pb en un gen transposasa presuntiva. A nivel de los ORFs 75, 94 y 125, se observó variación en la cantidad de secuencias repetitivas de 6 a 8, 1 a 3 y 2 a 13, respectivamente; esta variación permitió detectar hasta 8 genotipos de VSMB presentes en los langostinos de cultivo de la zona norte de Perú. El hallazgo inicial de estos genotipos representa un logro significativo que sentará las bases para futuros estudios epidemiológicos del VSMB en langostinos de cultivo y silvestres en esta región geográfica.
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Alfaro R., Guevara M. & Gonzales I. (2010). Prevalencia y distribución de los principales agentes etiológicos que afectan a los langostinos silvestres en Tumbes, Perú. Revista peruana de biología, 17(3), 359-364.
Chou, H., Huang, C., Wang, C., Chiang, H. & Lo, C. (1995). Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis. Aquat. Organ., 23(3), 165–173. https://doi.org/10.3354/DAO023165
Dieu, B., Vlak, J. & Zwart, M. (2011). Effects of extensive and intensive shrimp farming on the genetic composition of white spot syndrome virus populations, pp. 145-156. In: M. Bondad-Reantaso, J. Jones, F. Corsin y T. Aoki (Eds.). Diseases in Asian Aquaculture VII (pp. 145-156). Asian Fisheries Society, Fish Health Section, Selangor.
Dieu, B., Marks H., Zwart M. & Vlak J. (2010). Evaluation of white spot syndrome virus variable DNA loci as molecular markers of virus spread at intermediate spatiotemporal scales. Journal of General Virology, 91(5), 1164–78. https://doi.org/10.1099/vir.0.018028-0
Dieu, B. T. M, Marks, H., Siebenga, J., Goldbach, R., Zuidema, D., Duong, T. & Vlak, J. (2004). Molecular epidemiology of white spot syndrome virus within Vietnam. Journal of General Virology, 85(12), 3607–18. https://doi.org/10.1099/vir.0.80344-0
Flegel, T. (1997). Major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World Journal Microbiology Biotechnology, 13, 433–442. https://doi.org/10.1023/A:1018580301578
Hossain, S., Chakraborty, A., Joseph, B., Otta, S., Karunasagar, I. & Karunasagar, I. (2001). Detection of new hosts for white spot syndrome virus of shrimp using nested polymerase chain reaction. Aquaculture, 198(1-2), 1–11. https://doi.org/10.1016/S0044-8486(00)00571
Jiang, L., Xiao J., Liu L., Pan Y., Yan S. & Wang Y. (2017). Characterization and prevalence of a novel white spot syndrome viral genotype in naturally infected wild crayfish, Procambarus clarkii, in Shanghai, China. Virus Disease, 28(3), 250–261. https://doi.org/10.1007/s13337-017-0394-4
Li, F., Gao, M., Xu, L. & Yang, F. (2016). Comparative genomic analysis of three white spot syndrome virus isolates of different virulence. Virus Genes., 53, 249-258. https://doi.org/10.1007/s11262-016-1421-z
Lo, C. F., Leu, J. H., Ho, C. H., Chen, C. H., Peng, S. E. Chen, Y. T., Chou, C. M., Yeh, P. Y., Huang, C. J., Chou, H. Y., Wang, C. H. & Kou, G. H. (1996). Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Diseases of Aquatic Organisms, 25(1-2), 133–141. https://doi.org/10.3354/dao025133
Lo, C., Ho, C., Peng, S., Chen, C., Hsu, H., Chiu, Y., Chang, C., Liu, K., Su, M., Wang, C. & Kou, G. (1996a). White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Diseases of Aquatic Organisms, 27, 215–225. https://doi.org/10.3354/DAO027215
Marks, H., Goldbach, R., Vlak, J. & van Hulten, M. (2004). Genetic variation among isolates of white spot syndrome virus. Archives Virology, 149(4), 673–697. https://doi.org/10.1007/s00705-003-0248-9
Marks, H., Van Duijse, J., Zuidema, D., van Hulten, M. & Vlak, J. (2005). Fitness and virulence of an ancestral white spot syndrome virus isolate from shrimp. Virus Research, 110(1-2), 9–20. https://doi.org/10.1016/j.virusres.2005.01.002
Mayo, M. (2002). A summary of taxonomic changes recently approved by ICTV. Archives Virology, 147(8), 1655–1663. https://doi.org/10.1007/s007050200039
Min, J., Jeong, H. & Kim, K. (2023). Identification of Various indel-II variants of the white spot syndrome virus isolated from frozen shrimp and bivalves obtained in the Korean commercial market. Animals, 13(3348), 1-14. https://doi.org/10.3390/ani13213348
Muller, I., Andrade, T., Tang-Nelson, K., Marques, M. & Lightner, D. (2010). Genotyping of white spot syndrome virus (WSSV) geographical isolates from Brazil and comparison to other isolates from the Americas. Diseases of Aquatic Organisms, 88(2), 91–98. https://doi.org/10.3354/dao02142
Onihary, A., Razanajatovo, I., Rabetafika, L., Bastaraud, A. Heraud, J. & Rasolofo, V. (2021). Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016). Viruses, 13, 12-13. https://doi.org/10.3390/v13091713
Parrilla-Taylor, D., Vibanco-Pérez, N., DuránAvelar, M., Gomez-Gil, B., Llera-Herrera, R. & Vázquez-Juarez, R. (2018). Molecular variability and genetic structure of white spot syndrome virus strains from northwest Mexico based on the analysis of genomes. FEMS Microbiology Letters, 365(20), 1-9. https://doi.org/10.1093/femsle/fny216
Pereira, J., De Souza, E., Candido, J., Dantas, M., Nunes, A., Ribeiro, K., Teixeira, D. & Lanza D. (2019). Alternative PCR primers for genotyping of Brazilian WSSV isolates. Journal of Invertebrate Pathology, 162, 55-63. https://doi.org/10.1016/j.jip.2019.02.003
Pradeep, B., Shekar, M., Gudkovs, N., Karunasagar, I. & Karunasagar, I. (2008). Genotyping of white spot syndrome virus prevalent in shrimp farms of India. Diseases of Aquatic Organisms, 78(3), 189–198. https://doi.org/10.3354/dao01878
Ramírez, B., Guevara, M., Alfaro, R., Montoya, V., Mai, H., Serna, M., Dhar, A. & Aranguren, L. (2020). A cross-sectional study of shrimp pathogens in wild shrimp, Penaeus vannamei and Penaeus stylirostris in Tumbes, Peru. Aquaculture Research., 52(3), 1118-26. https://doi.org/10.1111/are.14969
Ramos-Paredes, J., Grijalva-Chon, J. & IbarraGamez, J. (2017). Virulence and genotypes of white spot syndrome virus (WSSV) infecting Pacific white shrimp Litopenaeus vannamei in north-western Mexico. Journal of Fish Diseases, 40(3), 425-35. https://doi.org/10.1111/jfd.12598
Restrepo, L., Reyes, A., Bajaña, L., Betancourt, I. & Bayot, B. (2018). Draft Genome Sequence of a White Spot Syndrome Virus Isolate Obtained in Ecuador. Genome Announcements, 6(26), 1-2. https://doi.org/10.1128/genomeA.00605-18
Rodrigues, E., Falcao, R., Guimaraes, G., Vinicios, B. & Miyoshi, S. (2020). Identification and genotyping of white spot syndrome virus (WSSV) isolated from Litopenaeus vannamei in the state Rio Grande do Norte. Brazilian Journal of Development, 6(7), 45379-45385. https://doi.org/10.34117/bjdv6n7-236
Shekar, M., Karunasagar, I. & Karunasagar, I. (2005). A computer based identification of variable number tandem repeats in white spot syndrome virus genomes. Current Science, 89(5), 882–887. https://www.jstor.org/stable/24111037
Tang, K., Groumellec, M. & Lightner, D. (2013). Novel, closely related, White spot syndrome virus (WSSV) genotypes from Madagascar, Mozambique and the Kingdom of Saudi Arabia. Diseases of Aquatic Organisms, 106(1), 1-6. https://doi.org/10.3354/dao02645
van Hulten, M., Witteveldt, J., Peters, S., Kloosterboer, N., Tarchini, R., Fiers, M., Sandbrink, H., Lankhorst, R. & Vlak, J. (2001). The white spot syndrome virus DNA genome sequence. Virology, 286, 7–22. https://doi.org/10.1006/viro.2001.1002
Vazquez-Boucard C., Alvarez-Ruiz P., EscobedoFregoso C., Anguiano-Vega G., Duran-Avelar M., Serrano V. & Escobedo-Bonilla C. (2010). Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas, Short Communication, Journal of Invertebrate Pathology, 104(3), 245-247. https://doi.org/10.1016/j.jip.2010.04.00
Vijayan K. K., Stalin Raj V., Balasubramanian C. P., Alavandi S. V., Thillai Sekhar V. & Santiago T. C. (2005). Polychaete worms a vector for white spot syndrome virus (WSSV). Diseases of Aquatic Organisms, 63(2-3), 107-111. https://doi.org/10.3354/dao063107
Wang, C., Lo, C., Leu, J., Chou, C., Yeh, P., Chou, H., Tung, M, Chang, C., Su, M. & Kou, G. (1995). Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Diseases of Aquatic Organisms, 23, 239–242. https://doi.org/10.3354/dao023239
Wang, Y., Lo, C., Chang, P. & Kou, G. (1998). Experimental infection of white spot baculovirus in some cultured and wild decapods in Taiwan. Aquaculture, 164(1-4), 221-231. https://doi.org/10.1016/S0044-8486(98)00188-4
Yang, F., He, J., Lin, X., Li, Q., Pan, D., Zhang, X. & Xu, X. (2001). Complete genome sequence of the shrimp white spot bacilliform virus. Journal of Virology, 75(23), 11811–11820. https://doi.org/10.1128/jvi.75.23.11811-11820.200
Zeng, Y. (2021). Molecular epidemiology of white spot syndrome virus in the world. Aquaculture, 537,1-6. https://doi.org/10.1016/j.aquaculture.2021.736509
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