Reproduction and larviculture of Larkinia grandis (Broderip & G. B. Sowerby, 1829) under laboratory conditions. Tumbes, Peru
DOI:
https://doi.org/10.53554/boletin.v40i2.435Keywords:
Larkinia grandis, conditioning, stimuli, pawning induction, larviculture, seedAbstract
Larkinia grandis is one of the key bivalve species inhabiting mangrove ecosystems, with significant ecological, economic, social, and culinary importance along the northern coast of Peru and in other countries within its distribution range across the Pacific Ocean. Due to the decline in its abundance index, mainly driven by fishing pressure, it is essential to develop controlled reproduction and cultivation methodologies to support population recovery strategies for the species. The aim of this study was to assess the reproductive potential of L. grandis, determine an effective spawning induction technique, obtain viable gametes, and conduct larviculture trials to produce seed in controlled environments. Spawning induction was achieved through a combination of desiccation and thermal shock stimuli. A total of 47.4% of broodstock responded to the induction protocol (44% males and 56% females), with spawning occurring on average 23 ± 5 hours after stimulation. Each female produced an average of 23.1 ± 5.8x106 oocytes. Fifteen minutes after fertilization, 65.7% of oocytes were successfully fertilized. The first veliger larvae were observed 15 hours’ post-fertilization. The larval culture period lasted 16 days, during which pediveliger larvae reached an average shell length (SL) of 264.5 ± 48.4 µm. Post-larval settlement survival reached 48.7%, with newly settled post-larvae measuring an average SL of 425.9 ± 58.4 µm after 20 days of cultivation post-fertilization. These results validate the feasibility of controlled reproduction of L. grandis and provide a foundation for aquaculture development and population enhancement initiatives.
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